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![Phosphate Buffer, pH 7.4 [0.2 M] - 500 ml](https://m.media-amazon.com/images/I/41rEx7siHhL._SS218_.jpg)
Item #:
145284312
Phosphate Buffer, pH 7.4 [0.2 M] - 500 ml
Item #:
145284312
€ 97
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Imported from USA store
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Manufactured in an ISO 9001 and 13485 certified facility in the USA, our buffers ensure the highest quality for your research and diagnostics.
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What Stands Out
Optimal pH Stability
Designed to maintain a consistent pH of 7.4, ensuring reliable results in biological and chemical applications, making it essential for laboratory environments.
High Concentration
With a concentration of 0.2 M, this buffer effectively neutralizes pH fluctuations, enhancing experiment accuracy compared to lower concentration alternatives.
Large Volume
The 500 ml supply provides ample buffer for extended experiments and multiple applications, reducing the need for frequent restocking and saving time in busy labs.
Product Details
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Laboratory Technicians
Essential for maintaining pH stability in biochemical experiments and assays in research laboratories.
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Cell Culture
Ideal for cell culture environments requiring a consistent pH for optimal cell growth and viability.
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Biochemical Analysis
Useful for various biochemical assays where pH 7.4 is crucial for accurate results and enzyme activity.
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Field Work
Not practical for outdoor applications where buffer conditions are difficult to maintain or monitor.
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Long-term Storage
Not suitable for long-term storage of solutions due to potential degradation and pH changes over time.
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Highly Alkaline Conditions
Not effective in situations requiring buffers for highly alkaline or acidic conditions outside of pH 7.4.
Product Description
Phosphate Buffer, pH 7.4 [0.2 M] - 500 ml
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Customer Questions & Answers
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Question:
What is Phosphate Buffer, pH 7.4 [0.2 M] used for?
Answer: Phosphate Buffer, pH 7.4 [0.2 M] is commonly used in biological and chemical applications to maintain a stable pH environment. A stable pH is crucial for many biochemical reactions, particularly in experiments involving enzymes, as they often require specific pH conditions to function optimally. This buffer can be utilized in cell culture, molecular biology assays, and various laboratory research settings. -
Question:
How should Phosphate Buffer, pH 7.4 be stored?
Answer: It is recommended to store Phosphate Buffer, pH 7.4 at room temperature away from direct sunlight and moisture. Proper storage ensures that the buffer retains its pH stability and does not degrade over time. For optimal use, avoid contamination by ensuring that the container is tightly sealed when not in use. -
Question:
Can I use Phosphate Buffer for cell culture?
Answer: Yes, Phosphate Buffer, pH 7.4 [0.2 M] is ideal for cell culture applications. It helps maintain physiological pH levels, which is essential for the growth and viability of cells in culture. This buffer is frequently used in the preparation of growth media for various types of cells, ensuring they thrive and function properly. -
Question:
Is Phosphate Buffer suitable for protein assays?
Answer: Absolutely! Phosphate Buffer, pH 7.4 is widely utilized in protein assays due to its ability to provide consistent pH conditions, which are necessary for accurate and reproducible results. Many protein purification and quantification methods, such as spectrophotometric assays, require a stable buffer environment to maintain protein structure and function. -
Question:
What are the components of Phosphate Buffer, pH 7.4?
Answer: Phosphate Buffer, pH 7.4 is composed of a mixture of disodium hydrogen phosphate (Na2HPO4) and sodium dihydrogen phosphate (NaH2PO4). This combination allows for buffering capacity at a pH of 7.4, making it an effective choice for biological and chemical applications. The balance of these components ensures that the buffer can resist changes in pH upon the addition of acids or bases. -
Question:
How is Phosphate Buffer prepared?
Answer: Phosphate Buffer, pH 7.4 can be prepared by mixing specific concentrations of Na2HPO4 and NaH2PO4 in distilled water. For accurate preparation, pH adjustments may be needed using a pH meter. This ensures that the desired pH is achieved and maintained. Laboratory technicians often prepare their buffers fresh to guarantee optimal performance in experiments. -
Question:
Can Phosphate Buffer be used in microbiology?
Answer: Yes, Phosphate Buffer, pH 7.4 is commonly used in microbiological studies. It serves as a diluent or a medium additive, providing a stable pH environment that is conducive for microbial growth. The buffer also plays a role in maintaining the integrity of microbial cell membranes during experimentation, thereby allowing for accurate assessments of microbial behavior. -
Question:
Is this buffer compatible with all types of samples?
Answer: Phosphate Buffer, pH 7.4 is compatible with a wide range of biological samples, including proteins, nucleic acids, and cells. However, certain sensitive reactions may require specific buffer conditions. Researchers are advised to consult relevant protocols to confirm the suitability of this buffer for their specific experiments, ensuring that their sample integrity is maintained. -
Question:
Where can I buy Phosphate Buffer, pH 7.4 [0.2 M] - 500 ml?
Answer: You can buy Phosphate Buffer, pH 7.4 [0.2 M] - 500 ml at Ubuy. Ubuy offers a convenient platform for purchasing laboratory reagents and chemicals, ensuring you can find high-quality products for your research or experimental needs.
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€ 97
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Secured transaction
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
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Features & Benefits
- Comprehensive range of biological buffers for various applications.
- ISO 9001 and 13485 certified manufacturing in the USA.
- Designed for protein research and molecular biology studies.
- Produced under stringent Good Manufacturing Practices.
- Ideal for immunochemical staining and preserving living tissues.
- Quality controlled in a certified clean room environment.